Vagus Nerve Visceral Ep Download Torrent

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On Friday (30), Vagus Nerve, a band featuring former God Forbid and current Meytal guitarist Doc Coyle, will release their debut EP, Visceral. The seven-track EP was successfully crowd-funded via Kickstarter, and you can listen to the whole thing here. This shift in baroreflex gain indicates that with repeated episodes of coherence, the activation. Visceral sympathetic afferent pain information to the brain (Foreman, 1989). It has been demonstrated that the cardiac branch of the vagus nerve.

Brain Behav Immun. Author manuscript; available in PMC 2015 May 1.
Published in final edited form as:
Published online 2013 Dec 25. doi: 10.1016/j.bbi.2013.12.015
NIHMSID: NIHMS560413
The publisher's final edited version of this article is available at Brain Behav Immun
See other articles in PMC that cite the published article.

Abstract

Mammals live in a co-evolutionary association with the plethora of microorganisms that reside at a variety of tissue microenvironments. The microbiome represents the collective genomes of these co-existing microorganisms, which is shaped by host factors such as genetics and nutrients but in turn is able to influence host biology in health and disease. Niche-specific microbiome, prominently the gut microbiome, has the capacity to effect both local and distal sites within the host. The gut microbiome has played a crucial role in the bidirectional gut-brain axis that integrates the gut and central nervous system (CNS) activities, and thus the concept of microbiome-gut-brain axis is emerging. Studies are revealing how diverse forms of neuro-immune and neuro-psychiatric disorders are correlated with or modulated by variations of microbiome, microbiota-derived products and exogenous antibiotics and probiotics. The microbiome poises the peripheral immune homeostasis and predisposes host susceptibility to CNS autoimmune diseases such as multiple sclerosis. Neural, endocrine and metabolic mechanisms are also critical mediators of the microbiome-CNS signaling, which are more involved in neuro-psychiatric disorders such as autism, depression, anxiety, stress. Research on the role of microbiome in CNS disorders deepens our academic knowledge about host-microbiome commensalism in central regulation and in practicality, holds conceivable promise for developing novel prognostic and therapeutic avenues for CNS disorders.

1. Introduction to microbiome

Human beings, like other mammals, live in a co-evolutionary association with huge quantities of commensal microorganisms resident on the exposed and internal surfaces of our bodies. The entirety of microorganisms in a particular habitat is termed microbiota, or microflora. The collective genomes of all the microorganisms in a microbiota are termed microbiome(; ). Commensal microbiota and microbiome outnumber human somatic cells and genome, respectively by approximately 10-100:1 (). The microbiota composition is influenced by temporal and spatial factors. Temporally, the human fetal gut is sterile but colonization begins immediately after birth and is affected by route of delivery, maternal transfer, diet, environmental stimuli and antibiotic usage (). However, the presence of bacteria has been detected in the meconium from healthy neonates, which might hint the existence of prenatal mother-to-child transfer of microbiota(; ). By 1 year of age, an idiosyncratic gut microbiome with adult-like signature is stabilized in each infant(). While adult gut bacterial communities vary, the concept of enterotype has been raised to classify individuals by their gut microbiota composition. Three enterotypes were characterized in human adults with relative abundance of Bacteroides, Prevotella or Ruminococcus genus(). Yet, discrete enterotypes are still arguable as a later study revealed gradients of key bacterial genera(). Whether human gut microbiota profiles fall into distinct clusters or a continuum depends on sampling strategy and methods of analysis and entails further comparison between healthy and diseased individuals.

Spatially, each body habitat is differentially dominated by specific phyla of microbiota: skin by Actinobacteria, Firmicutes and Proteobacteria; oral cavity by Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria; airway tract by Bacteroidetes, Firmicutes, and Proteobacteria; GI tract by Bacteroidetes and Firmicutes; and urogenital tract by Firmicutes (species under Lactobacillus genus)(). Adding to the complexity, there is an uneven spatial distribution of microbiota within each specific niche. In the human GI tract, the quantity and diversity of microbiota increase from stomach to small intestine and to colon(; ). Interestingly, microbiota have been identified within immune-privileged sites such as the CNS. α-proteobacteria class is reported to be the major commensals persistent in the human brain regardless of immune status().

While the host-microbiome interaction is not a novel concept, only recently has it been revisited by a surge of studies. Co-evolution has pre-determined that microbiota form a long-term symbiosis rather than short-term parasitism with human hosts. Yet, our prior and expanding knowledge about the effects of microbiome on host biology indicates that microbiota are not commensalistic bystanders that bring no benefit or detriment to hosts. Instead, a significant proportion of microbiota can be defined as symbionts or pathobionts, depending on whether they are mutualistic health-promoters or opportunistic pathology-inducers for hosts(). Host-microbiota mutualism is exemplary in the gut, where gut microbiome as a joint unity can be viewed as an organ of the host(). Traditionally, gut microbiome is considered to have three major categories of functions. First, it defends against pathogen colonization by nutrient competition and production of anti-microbial substances. Second, it fortifies intestinal epithelial barrier and induces secretory IgA (sIgA) to limit bacteria penetration into tissues. Third, it facilitates nutrient absorption by metabolizing indigestible dietary compounds. In line with these concepts, germ-free (GF) animals have higher susceptibility to infection but reduced digestive enzyme activities and muscle wall thickness(; ). Functional metatranscriptomic analysis of human fecal microbiota demonstrated a common pattern of overrepresented genes involved in carbohydrate metabolism, energy production and synthesis of cellular components ().

The recent trend of research has focused on the fourth role of gut microbiome: guiding maturation and functionality of the host immune system. Immune defects in GF mice are evident at both structural levels, such as decreased peyer's patches, lamina propria and isolated lymphoid follicles, and at cellular levels, such as decreased intestinal CD8+ T cells and CD4+ T helper 17 (Th17) cells and reduced B cell production of secretory IgA (sIgA)(). Th17 cells are potent mediators of mucosal immunity that produce signature cytokine IL-17, and sIgA is the principal immunoglobulin at mucosal sites that maintains barrier functions(; ). Other immune subsets, such as Foxp3+ regulatory CD4+ T cells (Tregs), invariant natural killer T (iNKT) cells and innate lymphoid cells (ILCs), are functionally affected by microbiota at pathological conditions(; ; ). Re-colonization of GF mice with a model gut commensal, Bacteroides fragilis, restored immune maturation at gut associated lymphoid tissues. Further, purified B. fragilis capsular polysaccharide A (PSA) was sufficient to expand splenic total CD4+ T cells and intestinal Foxp3+CD4 Tregs, which suggested that specific commensal antigens could drive immune regulation(; ). Gut microbiome provides diverse signals for tuning host immune status toward either effector or regulator direction, and is thus critical to peripheral immune education and homeostasis.

Microbiome at a specific niche can cast local as well as systemic effects on host biology. Disruption of a balanced composition of gut microbiome (termed dysbiosis) may cause chronic low-grade intestinal inflammation as seen in the irritable bowel syndrome (IBS) or intense intestinal autoimmunity as seen in the inflammatory bowel disease (IBD)(; ). Dietary change can bring symptomatic improvement in IBS patients. Moreover, gut microbiome alteration was observed in IBS patients, exemplified by the reduction of species under Lactobacillus genus and Clostridium class(; ). Similarly, IBD patients showed elevated antibody titers against indigenous bacteria, a drastic change of gut microbiome, and favorable response to antibiotic intervention(; ). Importantly, while genetic factors such as polymorphisms in NOD2 (nucleotide-binding oligomerization domain 2) influence susceptibility to IBD, animal studies show that dysbiosis alone suffice to induce IBD. Antibiotic depletion of microbiota cured intestinal inflammation in Tbx21-/-Rag-/- (TRUC) mice that lacked adaptive immunity and developed spontaneous IBD. Further, wild-type mice co-housed with TRUC littermates developed similar colitis symptoms(). Thus in the case of IBD, dysbiosis can directly lead to aberrant mucosal immunity, which in turn might maintain or exacerbate dysbiosis. On the other hand, beneficial gut bacteria can ameliorate IBD in both human studies and mouse models. Bifidobacteria, Lactobacillus and Bacteroides genera are the major components of beneficial probiotics(). Gut microbiota-derived products and metabolites, such as B. fragilis PSA and short-chain fatty acids (SCFA), also exerted potent anti-inflammatory functions in mouse IBD models(; ).

Systemically, gut microbiome contributes to the etiology of experimental disease models affecting remote organ systems. This can be caused by the trafficking of immune cells stimulated at the intestinal site, including microbe-sensing APCs and adaptive immune cells, to distal tissue sites, by systemic diffusion of commensal microbial products or metabolites, or by bacterial translocation as a result of impaired barrier integrity. At the liver sites, endotoxemia-induced inflammation is responsible for diseases such as cirrhosis(). At the airway mucosal sites, antibiotic modulation of gut commensals impaired protective anti-viral immunity during intranasal infection with influenza and systemic infection with lymphocytic choriomeningitis virus (LCMV)(; ). Gut microbiome influences various extra-intestinal autoimmune conditions as illustrated in murine models. Germ-free status confers a complete protection from spontaneous experimental autoimmune encephalomyelitis (EAE) and ankylosing spondylitis, a partial protection from spontaneous rheumatoid arthritis (RA) yet an enhanced level of spontaneous type-1 diabetes (T1D). Further, both GF and antibiotics-treated mice showed altered severity in inducible models of extra-intestinal autoimmune diseases(; ).

In this Review, we discuss the role of microbiome, especially gut microbiome, in relation to central nervous system (CNS) disorders. We analyze how microbiome liaises the bi-directional communication between gut and the critical distal site of CNS, and the mechanisms that guide each direction of function. We summarize the range of CNS disorders influenced by microbiome, which could be broadly classified into immune- and non-immune-mediated types. We further categorize the underlying microbiome-related factors implicated in CNS disorders. Our burgeoning knowledge about microbiome may provide novel avenues for therapeutics against neurological diseases.

2. Communication between gut microbiome and the CNS

The gut receives regulatory signals from the CNS and vice versa. The term gut-brain-axis thus describes an integrative physiology concept that incorporates all, including afferent and efferent neural, endocrine, nutrient, and immunological signals between the CNS and the gastrointestinal system(). As accumulating literatures underpin the importance of the gut microbiome to intestinal functions, a novel concept of microbiome-gut-brain axis has been evolved (). The core feature of this concept is bidirectional interaction, with diverse mechanisms guiding each direction of effects.

2.1. How the CNS influences microbiome

A classical CNS-gut-microbiome signaling is operational via central regulation of satiety. Changes of dietary pattern as a result of CNS control of food intake can impact nutrient availability to gut microbiota and consequently their composition. Satiation-signaling peptides are the key molecular intermediaries that enable this downward control. These peptides, for example peptide YY (PYY), are transported through blood to the brain postprandial to exert their impact on satiety (). Satiation-signaling peptides arise primarily from the GI tract but most of them are also synthesized within the brain (reviewed by ()). Beyond that, CNS can influence gut microbiome through neural and endocrine pathways in both direct and indirect manners. The autonomic nervous system (ANS) and hypothalamus-pituitary-adrenal (HPA) axis that liaise the CNS and viscera can modulate gut physiology such as motility, secretion and epithelial permeability as well as systemic hormones, which in turn affects the niche environment for microbiota and also host-microbiome interaction at the mucosae(). Santos et al. found that stress caused epithelial barrier defects and subsequent mucosal mast cell activation(). O'Mahony et al. illustrated that an early life stress (maternal separation) increased systemic corticosterone level and immune responses and altered fecal microbiota in rats(). Bailey et al. indicated that a social disruption (SDR) initiated by co-housing with aggressive male littermates altered murine gut bacterial populations through immune-activation(). Further, release of signaling molecules, cytokines, and anti-microbial peptides (AMPs) into the gut lumen by neurons, enteroendocrine cells, immune cells and Paneth cells at the direct or indirect command of the CNS is likely to have an immediate impact on gut microbiota(). Clarke et al. discovered the QseC sensor kinase as a bacterial receptor for host-derived epinephrine and norepinephrine, which might explain the biochemical basis for host endocrine signaling to microbiota().

2.2. How microbiome influences CNS functions

The influence of microbiome on CNS functions is manifested in both normal and disease conditions. There is a crucial link between gut microbiome and CNS maturation under physiological state. External cues derived from indigenous commensal microbiota affect prenatal and postnatal developmental programming of the brain(; ). On the other hand, co-morbidity with mood disorders such as depression and anxiety is common in the intestinal pathological state of IBS. Chronic low-grade inflammation or immune activation that underlies the etiology of IBS is also a driving risk factor in mood disorders(). In the more intense case of IBD, co-morbidity with stress is caused by the concurrent intestinal inflammation and microbiome alteration. Change in psychological activities is perceived in patients before and after IBD diagnosis().

Upward regulation of the CNS by microbiome can be achieved through neural, endocrine, metabolic and immunological mechanisms. The neural pathway is operational through the enteric nervous system (ENS), a main division of the ANS that governs the GI functions, and vagal afferent nerves (VAN) that convey sensory information from viscera to the CNS. Probiotic modulation of gut microbiota has been shown to influence gut neuro-motor functions(). Receptors expressed on VAN sense many of the regulatory gut peptides and also information contained in dietary components, relaying the signals to the CNS afterwards(). Indeed, vagal activation is necessary for a range of effects of gut microbiome or probiotics on brain functions(). Recent studies suggest a direct interaction between gut microbiome and enteric neurons. TLR-3, 7 (recognizing viral RNA) and TLR-2, 4 (recognizing peptidoglycan and lipopolysaccharide) are expressed by the ENS in both mice and human(; ). Kunze et al. observed that Lactobacillus reuteri enhanced excitability of colonic neurons in naïve rats by inhibiting calcium-dependent potassium channel(). Mao et al. found that ex vivo, both Lactobacillus rhamnosus (strain JB-1) and B. fragilis could activate intestinal afferent neurons, while PSA completely mimicked the neuronal effects of its parent organism B. fragilis(). Chiu et al. indicated that Staphylococcus aureus activation of sensory neurons could transduce nociception(). It is still unclear, in homeostatic periods, whether and how luminal microbial antigens reach into muscularis mucosa and sub-mucosa, where the ENS resides and the physical contact with sensory neurons occurs.

In the endocrinal pathway, the gut microbiome plays a major role in the development and regulation of the HPA axis that is critical to stress responses. Studies in gnotobiotic mice showed that postnatal exposure to gut microbiome affected the set point of the HPA axis(). Enteroendocrine cells interspersed among gut epithelium, particularly enterochromaffin cells, can secrete neurotransmitters and other signaling peptides in response to luminal stimuli, and thus act as transducers for the gut-endocrine-CNS route(). Besides, the vasoactive intestinal peptide (VIP), a peptide hormone synthesized in the gut but also brain, could mediate immune-modulation during CNS inflammation(). While the direct impact of microbiome on VIP expression has not been identified, dietary intervention is able to increase intestinal VIP, which might hint the role of microbiome().

Since a main function of microbiome is to facilitate host metabolism, a metabolic pathway is naturally implicit in the microbiome-gut-CNS signaling. Examples of metabolites associated with microbial metabolism or microbial–host co-metabolism have been reviewed(). Dysregulation of serotonergic and kynurenine routes of tryptophan metabolism influences the CNS pathological conditions of dementia, Huntington's disease and Alzheimer's disease(). Probiotic treatment could alter kynurenine levels and ameliorate CNS pathologies(). In addition, the metabolic pathway represents an important inter-kingdom communication as host signaling molecules can be fully synthesized or mimicked by microbiota-derived metabolites. Commensal organisms can produce a range of neuroactive molecules such as serotonin, melatonin, gamma-aminobutyric acid (GABA), catecholamines, histamine and acetylcholine(; ; ).

The immunological pathway seems to be an independent mechanism in the microbiome-gut-CNS signaling. The CNS, though viewed as an immune-privileged site, is not devoid of immune cells. There is a regular presence of macrophages and dendritic cells (DCs) in the choroid plexus and meninges, microglial cells in the brain parenchyma, and leukocytes in the cerebrospinal fluid (CSF). Aberrant CNS autoimmunity arises as a consequence of direct immune disruption of neural tissues. Commensal microbiome, known to shape the host immune system, affects the auto-reactivity of peripheral immune cells to the CNS(; ). Secondly, immune-to-CNS communication is also mediated by systemic circulation of immune factors, which is implicated in neuro-psychiatric disorders such as depression. Indeed, factors that increase peripheral inflammation markers such as C-reactive protein (CRP), IL-1, IL-6 and tumor necrosis factor (TNF-a), are also risk factors for depression(; ). In both routes of the pathway, there are anti-inflammatory mechanisms that can counter-act immune-mediated CNS disease symptoms.

3. The role of microbiome in CNS disorders

As multiple mechanisms guide the impact of microbiome on the CNS, it is therefore of particular interest to explore the role of microbiome in the regulation of CNS disorders. While there is still a lack of epidemiological evidence to connect microbiome with CNS pathologies, accumulating studies have underscored the importance of microbiome in a range of CNS disorders (). CNS disorders can be classified as immune-mediated (exemplified by CNS autoimmune diseases such as multiple sclerosis) and non-immune-mediated (exemplified by neuro-psychiatric disorders such as autism, depression, anxiety and stress) according to main etiologies. This dichotomy, however, is not arbitrary since there often exists a crosstalk of etiologies. We herein summarize how microbiome can affect both categories of CNS disorders.

3.1. How microbiome affects immune-mediated CNS disorders

3.1.1. Multiple sclerosis

Multiple sclerosis (MS) is a chronic CNS demyelinating disease mediated by auto-reactive immune attack against central neural tissues. EAE is a widely used animal model of MS induced by CNS-restrictive antigens. Although EAE might not recapitulate all the features of human MS, it simulates its core neuro-inflammation process(). Historically, viral infection, such as Epstein-Barr virus (EBV) or human herpes virus 6, has been suggested as the trigger for human MS(). Recent studies, however, have begun to elucidate the contribution of microbiome and its relevant factors to MS pathogenesis, with much of the work investigated in EAE models(). It has been shown in MOG92-106 TCR transgenic (RR) mice that commensal microbiota are essential for the development of spontaneous EAE. Germ-free RR mice were prevented from sEAE as a result of attenuated Th17 and auto-reactive B cell responses(). Commensal microbiota are also required for induced EAE model, as GF B6 mice developed less severe EAE accompanied with decreased IFN-γ and IL-17 responses and increased Foxp3+Tregs. Segmented filamentous bacteria (SFB) colonization restored EAE susceptibility in GF mice(). Antibiotic modulation of gut microbiota controls EAE progression via diverse cellular mechanisms. Ochoa-Reparaz et al. demonstrated that IL-10-producing CD4+CD25+Foxp3+Tregs were required for oral antibiotic attenuation of EAE progression(). In a following study, Ochoa-Reparaz et al. showed that oral antibiotic treatment of EAE mice systemically induced a regulatory CD5+B cell subset(). Yokote et al. found that iNKT cells, a CD1d-restricted T cell subset that shared properties of both T and NK cells, were necessary for oral antibiotics amelioration of murine EAE (). While it is unknown whether enteric microbiota affect human MS, a higher percentage of MS patients exhibited antibody responses against gastrointestinal antigens in contrast to healthy control, which could indicate altered gut microbiome and immune status().

Oral treatment with a single bacterium or bacteria mixture can modulate EAE as observed in a range of studies. Probiotic Bifidobacterium animalis reduced the duration of symptoms in a rat EAE model(). Conversely, probiotic strain Lactobacillus casei Shirota (LcS) exacerbated EAE symptoms in rats(). However, later studies indicated that probiotic Lactobacilli, inclusive of LcS, did not enhance but rather suppressed rat EAE(). This has been corroborated by other studies using probiotic mixtures of strains under the Lactobacillus genus. Indeed, Lactobacilli (including LcS), either administrated alone or in combination with other strains of Bifidobacterium genus, tend to alleviate murine EAE symptoms via reciprocal regulation of pro- and anti-inflammatory cytokine responses(; ; ; ). Probiotic treatment with B. fragilis and Pediococcus acidilactici (strain R037) also significantly reduced mice susceptibility to EAE(; ). In the case of the human commensal B. fragilis, capsular PSA expression was critical for its immune-regulatory functions(). Further, engineered strains such as Salmonella-CFA/I and Hsp65-producing Lactococcus lactis can prevent EAE in mice via Tregs-associated TGFβ and IL-13 signals(; ; ).

Vagus

Isolated commensal microbial products can often recapitulate the biological effects of their parent organisms on hosts. Some of these products have been found as potent therapeutics against EAE. Purified B. fragilis PSA, referred to as a symbiosis factor in other studies, conferred prophylactic as well as therapeutic protection against EAE via induction of tolerogenic CD103+DCs at CNS-draining lymph nodes, similar to the effects conferred by probiotic B. fragilis(). While PSA is a TLR2 ligand, its immune-regulatory functions against EAE are not seen as putative in other commensal-derived TLR2 ligands. Nichols, et al. reported that a unique lipid TLR2 ligand, phosphorylated dihydroceramide (PE DHC), derived from human oral commensal Porphyromonas gingivalis but also gut commensals, was able to exacerbate murine EAE via TLR2-dependent mechanisms(). Commensal-derived extracellular ATP can be viewed as a danger-associated molecular pattern (DAMP) by hosts and has been related to Th17 development. Accordingly, Entpd7-/- mice that are deficient of ATP hydrolyzing enzymes have displayed a more severe level of EAE().

Finally, diet patterns have been reported to influence the development of EAE. Piccio et al. found that high-fat diet increased murine EAE severity. In contrast, calorie restriction diet attenuated EAE symptoms, which was associated with hormonal, metabolic and cytokine changes rather than immune suppression(). Kleinewietfeld et al. illustrated that mice fed with a high-salt diet developed a more severe form of EAE, in line with the ability of sodium chloride to activate Th17 cells(). Recent developments may insinuate a central role of gut microbiome in linking diet with MS and EAE.

3.1.2. Neuromyelitis optica

Neuromyelitis optica (NMO), also known as Devic's disease, is a CNS autoimmune disease featured by immune-mediated demyelination of the optic nerve and spinal cord. It resembles multiple aspects of MS. Auto-reactive humoral and T cell-mediated immunity against aquaporin 4 (AQP4), a predominant CNS water channel protein, drives the NMO pathogenesis(; ). Like MS, no research so far has established a direct link between gut microbiome and NMO. Banati et al. found that patients of AQP4-seropositive NMO and NMO spectrum diseases showed much higher serum level of antibodies against gastrointestinal antigens (most frequently dietary proteins) than did healthy controls, insinuating the alteration of microbiota composition and consequent immune status in NMO patients(). Varrin-Doyer et al. found that AQP4-specific T-cells in NMO patients showed cross-reactivity to a protein of the indigenous gut commensal species, Clostridium perfringens, supporting a microbiota-related molecular mimicry process in NMO pathogenesis().

3.1.3. Guillain–Barré syndrome

Guillain–Barré syndrome (GBS) is an autoimmune disease of the peripheral nervous system. Similar to MS, auto-reactive immune attack of myelin acts as the cause of neuro-degeneration in GBS(). Preceding infection with bacteria or virus, such as Haemophilus pneumoniae, Mycoplasma pneumoniae, influenza, and EBV, has been suggested as environmental triggers for GBS. Indeed, cross-reaction of pathogen-induced antibodies against neural surface antigens in a molecular mimicry process constitutes an important mechanism for GBS neuronal damage that leads to acute flaccid paralysis(). Campylobacter jejuni, a gut commensal species found in poultry, is a major cause of human enteritis induced by food contamination. Tam et al. indicated a far greater risk of GBS among Campylobacter enteritis patients than previously reported by retrospective serological studies(). Further, Campylobacter is associated with several pathologic forms of GBS. Different strains of Campylobacter, along with host factors, play an important role in shaping auto-reactive immune reactions during GBS development(). Therefore, C. jejuni represents a gut-associated pathogen that mediates neural autoimmunity.

3.1.4. Other immune-mediated conditions

The role of microbiome has been implicated in other immune-involved CNS diseases. Meningitis is inflammation of the protective membranes of the CNS. Viral or bacterial infection may lead to meningitis. Zelmer et al. reported that the adult gut commensal Escherichia coli K1 were able to cause meningitis via maternal transfer to newborn infants. The polysialic acid (polySia) capsule synthesized by E. coli K1 guided the critical process of blood-to-brain transit of this neuro-pathogenic strain(). Chronic fatigue syndrome (CFS), also referred to as myalgic encephalomyelitis (ME), is so far of unknown etiology. Immune factors, such as chronic lymphocyte over-activation and cytokine abnormalities, contribute to its pathogenesis(). Maes et al found that increased IgA responses to commensal bacteria in CFS patients were associated with inflammation, cellular immune activation, and symptomatic severity. It was postulated that elevated translocation of commensal bacteria could be responsible for the disease activities in some CFS patients().

3.2. How microbiome affects non-immune-mediated CNS disorders

3.2.1. Autism and depression

Autism spectrum disorder (ASD) is a range of developmental neuro-behavioral disorders characterized by impaired social interaction and communication. Autism represents the primary type of ASD. Emerging data have indicated a link between gut microbiome and ASD, either as direct causality or as indirect consequences of atypical patterns of feeding and nutrition(). Disruption of gut microbiota might promote the over-colonization of neurotoxin-producing bacteria and thus contribute to autistic symptoms. It has been reported, however, that oral vancomycin treatment brings short-term benefit to regressive-onset autism children(). General gut microbiota alteration or specific gut commensal strains have been implicated in ASD. Bolte et al. postulated that Clostridium tetani could induce autism(). Indeed, two ensuing human gut microbiome studies illustrated a greater number of species under the Clostridium genus present in fecal samples of autistic children(; ). An imbalance of Bacteroidetes and Firmicutes phyla also manifests in autistic children. Finegold et al. reported increased presence of Bacteroidetes in severe autistic group and predominant presence of Firmicutes in healthy controls(). Williams et al. revealed a reverse trend in comparing autism and GI disease co-morbid (AUT-GI) children and GI disease alone controls(). In addition, altered levels of other gut commensals, including those of Bifidobacterium, Lactobacillus, Sutterella, Prevotella and Ruminococcus genera and of the Alcaligenaceae family, were correlated with autism(; ; ; ). Nonetheless, there are studies refuting the microbiota alteration between autistic and healthy subjects(). Variance in sampling strategies and techniques applied to microbiome assays may account for these differences. Further, gut microbiome-mediated metabolism also impacts autism. Metabolites profile gathered from both urinary and fecal samples differed in autistic patients and healthy control, potentially consequent of microbiota changes(; ; ).

Depression is a major form of mood disorder that results from neuro-psychiatric disturbance or immunological deregulation(). Probiotic treatment has shown efficacy in suppression of animal depression models. Species under Lactobacillus genus are particularly characterized as anti-depressant. Probiotic mixture comprising L. rhamnosus and L. helveticus strains ameliorated maternal separation-induced depression via normalizing corticosterone level(). Similarly, L. rhamnosus strain JB-1 reduced depression-related behavior through regulating corticosterone and GABA receptor in a vagal-dependent manner(). Species of Bifidobacterium are also potent anti-depressants. Bifidobacterium infantis alleviated depression as indicated by rat forced swim test (FST) and maternal separation models. Mechanisms involved include attenuation of pro-inflammatory cytokines, regulation of tryptophan metabolism and CNS neurotransmitters(; ). Probiotics combining Lactobacilli and Bifidobacteria were tested in post-myocardial infarction depression models. L. helveticus and Bifidobacterium longum together ameliorated post-MI depression through reduction of pro-inflammatory cytokines and restoration of barrier integrity at GI tract(; ). In addition, gut microbial products, such as sodium butyrate (salt formed from butyrate acid, a type of SCFA) have been explored in animal depression model, without showing anti-depressant effects(). Further, a diet formulation containing high levels of polyunsaturated fatty acids (PUFAs) n-3 attenuated rat post-MI depression via similar mechanisms as did L. helveticus and B. longum().

3.2.2. Anxiety and stress

Anxiety and stress are common forms of mood disorders with nervous, endocrinal and immunological basis. Exposure to stressors such as chemical, biological or environmental stimuli can trigger stress and anxiety responses, which involves activation of the HPA axis. As aforementioned, co-morbidity with anxiety and stress has been perceived in drastic and mild types of intestinal dysfunctions, underscoring the role of gut-brain signals such as neurotransmitters and immune factors(; ; ; ; ; ).

GF mice showed increased motor activity and reduced anxiety, compared to SPF mice with normal gut microbiota. This behavioral phenotype was associated with higher levels of neurotransmitters and reduced synaptic long-term potentiation in the CNS of GF mice(). Reduced anxiety-like behavior in GF condition has been confirmed by later studies, which are explained by other neurochemical changes such as decreased neurotransmitter receptors and increased tryptophan metabolism. It is therefore postulated that gut microbiome regulates the set point for HPA axis(; ). Gut-associated pathogens can exacerbate anxiety. Infection with C. jejuni elevated anxiety-like behavior through induction of the c-Fos protein, a neuronal activation marker, in the CNS as well as ANS(; ). C-Fos protein induction was also indicated in Citrobacter rodentium exacerbation of anxiety, whereas Trichuris muris elevated anxiety via immunological and metabolic mechanisms(; ). In contrast, beneficial probiotics can ameliorate anxiety. Specific species of Lactobacillus and Bifidobacterium genera have anxiolytic effects. Probiotic treatment with certain strains of B. longum, B. infantis, L. helveticus, or L. rhamnosus, either alone or in combination, normalized behavioral phenotypes in animal anxiety models(; ; ; ; ).

Programming of HPA axis by gut microbiome is also observed in stress condition. GF mice showed exaggerated HPA stress response, accompanied by increased circulatory neurotransmitters and decreased brain-derived neurotrophic factor (BDNF) expression in the CNS(). Altered gut microbiota composition has been associated with stress. O'Mahony, et al. reported changes in fecal microbiota in early life stress induced by maternal separation(). Murine exposure to the SDR stressor led to decreased abundance of Bacteroides, increased abundance of Clostridium, and changes of other bacteria genera, which were concurrent with enhanced circulatory pro-inflammatory cytokines(). The anxiolytic strains of Lactobacillus and Bifidobacterium genera that have anti-anxiety effects often display anti-stress effects as well. Ingestion with L. helveticus and L. rhamnosus reduced rat chronic psychological stress indicated by water avoidance test and improved intestinal barrier integrity(). Lactobacillus farciminis also suppressed stress-induced gut leakiness and attenuated HPA axis stress response(). B. longum normalized anxiety-like behavior and CNS BDNF levels in mice co-morbid with infectious colitis through a vagal-dependent mechanism(). A probiotic formulation consisting of L. helveticus and B. longum showed anxiolytic-like activities in rats and beneficial psychological effects in healthy human subjects().

3.2.3. Pain

Nociceptive pain that is caused by peripheral nervous response to stimuli and signaling transduction to the CNS can be alleviated by probiotic modulation of microbiome. Antinociceptive effects are seen in species of Lactobacillus genus. L. farciminis ameliorated stress-induced hypersensitivity to colorectal distension (CRD), mediated by inhibition of colonic epithelial contraction and nitric oxide (NO)-related mechanisms(). L. reuteri also attenuated visceral pain induced by CRD in normal rats(). L. paracasei normalized visceral hypersensitivity to CRD in antibiotics-perturbed mice (). Lactobacillus acidophilus delivered analgesic effects in intestinal pain via induction of opioid and cannabinoid receptors(). Besides, two studies supported the anti-nociceptive effects of a specific B. infantis strain in the context of IBS. Probiotic B. infantis reduced CRD-induced pain in both visceral normal-sensitive and visceral hypersensitive rat strains, and also in a rat model of post-inflammatory colonic hypersensitivity(; ). Recently, Chiu et al. reported that S. aureus triggered pain in mice through direct induction of calcium flux and action potentials in nociceptor neurons().

3.2.4. Other neuro-psychiatric symptoms

Microbiome has been connected with other neuro-psychiatric disorders, where a mixture of immune- and non-immune-based etiologies often occurs. GF animals exhibit defective memory and cognitive abilities. Gareau et al. found that memory dysfunction occurred in GF mice regardless of exposure to stress(). Bercik et al. showed that re-colonization of GF mice with murine microbiota could either enhance or reduce exploratory behavior, depending on the strains of donor and recipient mice. Further, antibiotic treatment of SPF mice increased exploratory behaviors. Hippocampal levels of BDNF were positively correlated with exploratory behaviors, and regulated in both cases(). Probiotics were able to improve infection-induced memory dysfunction and diabetes-induced cognitive defects(; ). Propionic acid, a type of SCFA, reduced murine social and cognitive abilities(). Dietary alteration of gut microbiome also modulated murine cognitive and learning behaviors(). Microbiota alteration has been indicated in hepatic encephalopathy (HE). Different fecal and mucosal microbiota were found in HE patients as compared to healthy controls. In cirrhotic HE specifically, good cognition and decreased inflammation were linked with autochthonous and Prevotella genera as well as Alcaligenaceae and Porphyromonadaceae families, whereas poor cognition and increased inflammation were linked with over-represented Enterococcus, Megasphaera and Burkholderia genera(; ; ). Alteration of serum antibodies to oral microbiota and sub-gingival bacterial species was observed in Down's syndrome(; ). Oral microbiota changes were also observed in comatose patients(). A positive correlation between schizophrenia and serological surrogate markers of bacterial translocation was indicated().

Vagus Nerve Visceral Ep Download Torrent 1

4. Factors linking microbiome and the CNS

As microbiome refers to the collective genomes of total microbiota, microbiome research is broad in its scope, which incorporates general microbiota composition or specific bacterium, microbiota-generated products, external alteration of microbiota, and barrier integrity status that affects host-microbiota contact. It is thus worthy summarizing the factors that mediate the influence of microbiome on CNS disorders.

4.1. Hygiene

The hygiene hypothesis states that a lack of childhood exposure to infectious agents, parasites and commensals increases susceptibility to T helper 2 (Th2)-mediated allergic diseases. However, there also exists a correlation between improved sanitary conditions and increased incidences of T helper 1 (Th1)-mediated autoimmune diseases such as T1 diabetes and multiple sclerosis(). Th1 response targets intracellular microbes, mediated by signature cytokine IFNγ; while Th2 response targets helminthes and allergens, characterized by signature cytokines IL-4 and IL-13. Aberrant immune development is therefore a potential mechanism that links hygiene and immune-mediated CNS disorders. GF mice displayed reduced EAE symptoms, concurrent with attenuated Th1, Th17 and B cell responses, which related to the hygiene hypothesis yet contradicted findings in human MS(; ). This discrepancy might be explained by intricate etiologies underlying human MS and intrinsic differences between murine GF condition and human hygienic state. In murine models, GF condition is also linked to neuro-behavioral disorders. Total sterility results in reduction of BDNF levels and enhancement of HPA axis responses, correlated by elevated neurotransmitters in the plasma. GF animals displayed increased stress and impaired cognition(; ). However, GF condition in other studies is identified as anxiolytic and can resolve anxiety, correlated by decreased neurotransmitter receptors levels(; ). Hence, hygiene exerts case-specific rather than universal influences on neuro-chemistry and neuro-behavioral manifestations.

4.2. Antibiotics usage

Antibiotics confer selective alteration of gut microbiota. Mice pre-conditioned with oral antibiotics are less susceptible to autoimmune models such as EAE. In studies conducted by Ochoa-Reparaz et al., amelioration of EAE was associated with reduced IFNγ and IL-17, increased IL-13 and IL-10, and systemic stimulation of Tregs and Bregs(; ). That antibiotics poise the Th1/Th2 equilibrium towards Th2 direction is consistent with hygiene hypothesis. An earlier study conducted by Yokote et al. also observed reduced pro-inflammatory cytokines, including IFNγ and IL-17, in antibiotic treatment of EAE. While iNKT cells were not induced by antibiotics, they were essential for protection against EAE(). Different antibiotic agents were utilized in these EAE studies, which could result in different gut microbiome profiles and explain the variability of immune mechanisms. Current studies support a beneficial role of antibiotic treatment of neuro-behavioral disorders. Antibiotic treatment reduced stress response and increased exploratory behavior in mice and offered short-term benefit to regressive-onset autism children. Underlying mechanisms may involve the reduction of luminal LPS concentration (and thus potentially reduced chronic inflammation) and changes of CNS signals, such as hippocampal expression of BDNF(; ; ). In sum, antibiotics might reset the default immune and neuro-hormonal status shaped by commensal microbiome and therefore alter predisposition to CNS disorders.

4.3. Microbiota composition

How microbiota composition impacts CNS disorders can be indicated by a variety of methodologies, including infection-induced microbiome perturbation, studies using SPF and gnotobiotic mice, mono-colonization of GF mice, and metagenomic approaches such as microbial microarray and 16S rRNA profiling. Further, compositional changes of microbiota can be indirectly reflected by profiling the metabolites and co-metabolites of microbiota and serum titers of antibodies against microbiota and diet components. As the study of enterotypes is still in its infancy, efforts to find disease-specific enterotypes are limited. Hildebrand et al. defined two murine enterotypes, ET1 and ET2 that bore striking similarity to Ruminococcus and Bacteroides enterotype in human, respectively. ET2 mice showed higher levels of fecal calprotectin, a biochemical marker for IBD(). For CNS disorders, a concrete link with enterotypes has yet to be established. While it is tempting to infer enterotypes from the scattered studies of certain disease type, opposing data often obstruct consensus. For instance, there are favorable and unfavorable results for the link between Bacteroides enterotype and autism(; ). Further, heed must be taken to clarify the cause and effect as CNS disorders could impact diet patterns or be concurrent with gut epithelial impairment, both scenarios affecting microbiota composition.

4.4. Probiotics

Ingestion of beneficial live bacteria, also know as probiotics, is a therapeutic way of using microbiota components for treatment. Probiotics can regulate immune subsets, especially in the case of CNS autoimmunity. B. fragilis is a prominent probiotic strain that promotes Foxp3+Treg quantity and functional maturation in both EAE and IBD(; ). Lactobacilli and Bifidobacteria are key components of anti-inflammatory probiotic mixtures that can also function through stimulation of IL-10+Foxp3+Tregs(; ). Moreover, genetic modification of natural strains represents another potent probiotic approach. Fusing tolerogenic antigen into attenuated or innocuous strains has yielded oral therapeutics against EAE(; ; ). Probiotics can alleviate neuro-psychiatric disorders via hormonal and neuro-chemical mechanisms. For example, B. longum NCC3001 can normalize murine hippocampal BDNF expression and L. rhamnosus (JB-1) can exert differential regulation of GABA transcription in different CNS regions(; ). Particular probiotics may convey anxiolytic effects in multiple types of neuro-behavioral disorders, which indicates shared neural and endocrinal etiologies of these disorders. For example, L. helveticus R0052 and B. longum R0175 can ameliorate both anxiety and depression in rats(; ). Neural mechanisms that involve direct bacterial activation or inhibition of neurons may account for anti-nociceptive effects of probiotics.

4.5. Microbiota-derived products

Microbiota-derived products are often effective components responsible for microbiota-gut-CNS signaling. This is especially evident in the case of B. fragilis capsular PSA, where PSA can recapitulate the functions of its parent organism B. fragilis in regard to anti-inflammatory effects in EAE and activation of intestinal sensory neurons. PSA is a unique zwitterion and referred to as a symbiosis factor for commensalism(; ). Commensal-produced luminal extracellular ATP and LPS drive the chronic inflammation that contributes to the pathogenesis of neuro-immune and neuro-psychiatric disorders. Microbiota-derived metabolites and co-metabolites are critical intermediaries for microbiota-gut-CNS signaling. Commensals spawn a range of neuro-active substances. For example, Lactobacillus and Bifidobacterium species can produce the inhibitory neurotransmitter GABA(). The involvement of neuro-active metabolites in probiotic effects on neuro-psychiatric disorders remains unexplored. SCFAs, a group of fatty acids with aliphatic tails of 2 to 6 carbons, are fermentation products of dietary fibers by microbiota. While SCFAs have been found to be important immune regulators, there is a scarcity of studies that target at their impacts on CNS disorders(; ).

4.6. Diet

Diet patterns may modulate gut microbiome via alteration of nutrient availability. Recent developments have suggested that dietary intervention can impact gut microbial gene richness. Lower microbiome richness was identified as less healthy and associated with metabolic dysfunction and low-grade inflammation. Dietary formula with higher fiber contents can improve microbiome richness(; ). Unhealthy diet patterns containing high levels of fat or salt could accelerate neuro-inflammation during EAE(; ). Western-style diet could negatively affect anxiety-like behavior and memory, depending on immune status(). Supplementation with high levels of PUPAs could alleviate depression(). These experimental findings could indicate saturated fat as a risk factor for both neuro-immune and neuro-psychiatric disorders. Collectively, microbiome modulation is an integral mechanism underlying diet-based treatment.

4.7. Gut permeability

Gut permeability has been directly and indirectly associated with the role of microbiome in CNS disorders. Humoral and cellular immune reaction to microbiota in the circulation, persistent low-grade inflammation and neuro-psychiatric co-morbidity with IBD may hint the breach of mucosal epithelial barrier(; ; ; ; ; ). Probiotic treatment with several species of Lactobacillus genus restored the barrier integrity(; ). Dysbiosis and breakdown of mucosal barrier are interrelated phenomena. Microbiota and their ligands maintain the cell-cell junctions critical to barrier integrity(; ). Abnormal gut microbial composition is seen in IBD(). In return, the cascade of inflammatory process during IBD may amplify intestinal dysbiosis. Although it is hard to determine the initial cause, dysbiosis and gut hyper-permeability orchestrate in driving CNS pathogenesis.

5. Conclusions and perspectives

Accumulating information of animal and human research strengthen the concept of microbiome-gut-brain axis. Microbiome controls canonical aspects of the CNS, immunity and behavior in health and disease. Still, unknowns abound regarding the detailed role of microbiome in CNS disorders. First, the relative contributions of immune, neural, and endocrine pathways in microbiome-CNS communications at pathological states need to be clarified. Second, it is crucial to elucidate the factors at play in microbiome-based therapeutics and further refine the effective components. Third, caution should be applied to the translation of animal data to human clinics using existing microbiome studies.

Microbiome research holds conceivable promise for the CNS disorder-relevant prognosis and therapeutics. Correlational studies that associate microbiota compositional patterns with specific disorders such as autism types contain prognostic value. Multitudes of commensal bacteria co-exist with hosts without incurring harmful immune responses. Symbiotic strains and their products are thus a precious mining pool that contains useful drug candidates with host-tolerated immune-modulatory functions. Innocuous commensal strains could also act as carriers for therapeutic substances when engineered. Finally, to restore the richness and functionality of gut microbial ecosystem by fecal transplantation has been proposed long time ago yet methodological and ethical obstacles remain.

​ Microbiome-gut-brain axis in relation to CNS disorders

Multiple pathways guide the downward and upward directions of the microbiome-gut-brain axis in the contexts of health and disease. (A) Downwardly, CNS controls gut microbiome composition through satiation signaling peptides that affect nutrient availability, endocrines that affect gut functions and neural pathways. HPA axis release of cortisol regulates gut movement and integrity. Immune (cells, cytokines and sIgAs) pathways can be turned on in response to altered gut functions. Endocrine and neural pathways can also regulate the secretion from specialized gut epithelial cells, including paneth cells, enteroendocrine cells (ECC) and goblet cells. Their secretory products affect the survival and resident environment of microbiota. (B) Upwardly, gut microbiome controls CNS activities through neural (direct activation of neurons by microbiome), endocrine (e.g. ECC release of 5-HT), metabolic (microbiota synthesis of neuroactive molecules), and immune (CNS infiltrating immune cells and systemic inflammation) pathways. Microbiome influences CNS at healthy (neuro-development) and disease (a range of neuro-immune and neuro-psychiatric disorders) states. Gut luminal microbiota, their products sampled by APCs and epithelium-attaching SFBs mediate peripheral immune education. Gut microbiome composition, specific strains within microbiota, probiotic treatment, microbiota-derived products and other factors constitute the scope of microbiome studies.

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Acknowledgments

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We thank Dr. Pamela Bagley (Dartmouth College) for literature assistance.

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Abbreviations

AMPsanti-microbial peptides
TPHtryptophan
5-HT5-hydroxytryptamine
SFBSegmented filamentous bacteria
PSApolysaccharide A from B. fragilis
ATPadenosine-5′-triphosphate
SCFAshort-chain fatty acid
IECintestinal epithelial cell
ILCsinnate lymphoid cells
APCantigen presenting cell
MSmultiple sclerosis
NMOneuromyelitis optica
GBSGuillain-Barré syndrome
ASDautism-spectrum disorder
IBSirritable bowel syndrome
IBDinflammatory bowel disease

Footnotes

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References

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